Achieving super-resolution fluorescence imaging with focused spot illumination and non-negative inversions (Conference Presentation)

Jiun-Yann Yu; Stephen R Becker; James Folberth; Bruce F Wallin; Simeng Chen; V V Narumanchi; Jian Xing; Carol J Cogswell

doi:10.1117/12.2306354

14 March 2018 Achieving super-resolution fluorescence imaging with focused spot illumination and non-negative inversions (Conference Presentation)

Jiun-Yann Yu, Stephen R Becker, James Folberth, Bruce F Wallin, Simeng Chen, V V Narumanchi, Jian Xing, Carol J Cogswell

Author Affiliations +

Proceedings Volume 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV; 1049907 (2018) https://doi.org/10.1117/12.2306354
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

We demonstrate a new method for two-dimensional super-resolution fluorescence imaging. Our method achieves more than 2-fold resolution enhancement over the Rayleigh resolution limit simply with a focused illumination spot and non-negative least-squares inversions, and with practical photon budgets that can be supported by common fluorophores used for biological fluorescence microscopy.

Conference Presentation

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Jiun-Yann Yu, Stephen R Becker, James Folberth, Bruce F Wallin, Simeng Chen, V V Narumanchi, Jian Xing, and Carol J Cogswell "Achieving super-resolution fluorescence imaging with focused spot illumination and non-negative inversions (Conference Presentation)", Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 1049907 (14 March 2018); https://doi.org/10.1117/12.2306354

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