In vivo Brillouin microscopy of the larval zebrafish spinal cord (Conference Presentation)

Raimund Schlüßler; Stephanie Möllmert; Jürgen Czarske; Jochen Guck

doi:10.1117/12.2289949

15 March 2018 In vivo Brillouin microscopy of the larval zebrafish spinal cord (Conference Presentation)

Raimund Schlüßler, Stephanie Möllmert, Jürgen Czarske, Jochen Guck

Proceedings Volume 10496, Optical Elastography and Tissue Biomechanics V; 104960T (2018) https://doi.org/10.1117/12.2289949
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

The mechanical properties of biological tissues are increasingly recognized as crucial parts of signaling cascades involved in developmental and pathological processes. While most techniques measuring intrinsic mechanical properties necessitate invasive sample preparations or are currently applicable only to large sample dimensions, confocal Brillouin microscopy provides means to quantify the mechanical properties of single cells and tissues in a contact- and label-free manner. Here, we show for the first time a systematic application of confocal Brillouin microscopy to quantify physical properties of tissues in vivo. By using our custom-built Brillouin microscope, zebrafish larvae were probed in all anatomical planes, at different time points during development and after spinal cord injury. These experiments revealed that confocal Brillouin microscopy is capable of detecting the mechanical properties of distinct anatomical structures without interfering with the animal’s natural development. We furthermore detected an increasing Brillouin shift of spinal cord tissue during development and a transiently decreasing Brillouin shift after spinal cord injury. The presented work constitutes the first step towards an in vivo assessment of spinal cord tissue mechanics during regeneration, provides a basis to identify key determinants of mechanical tissue properties and allows to test their importance in combination with biochemical and genetic factors.

Conference Presentation

Citation Download Citation

Raimund Schlüßler, Stephanie Möllmert, Jürgen Czarske, and Jochen Guck "In vivo Brillouin microscopy of the larval zebrafish spinal cord (Conference Presentation)", Proc. SPIE 10496, Optical Elastography and Tissue Biomechanics V, 104960T (15 March 2018); https://doi.org/10.1117/12.2289949

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KEYWORDS

Spinal cord

Tissues

Confocal microscopy

Microscopy

In vivo imaging

Injuries

Signal processing

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