Optically enhanced acoustophoresis

Craig McDougall; Paul O'Mahoney; Alan McGuinn; Nicholas A. Willoughby; Yongqiang Qiu; Christine E. M. Demore; Michael P. MacDonald

doi:10.1117/12.2276323

25 August 2017 Optically enhanced acoustophoresis

Craig McDougall, Paul O'Mahoney, Alan McGuinn, Nicholas A. Willoughby, Yongqiang Qiu, Christine E. M. Demore, Michael P. MacDonald

Author Affiliations +

Proceedings Volume 10347, Optical Trapping and Optical Micromanipulation XIV; 103471B (2017) https://doi.org/10.1117/12.2276323
Event: SPIE Nanoscience + Engineering, 2017, San Diego, California, United States

Abstract

Regenerative medicine has the capability to revolutionise many aspects of medical care, but for it to make the step from small scale autologous treatments to larger scale allogeneic approaches, robust and scalable label free cell sorting technologies are needed as part of a cell therapy bioprocessing pipeline. In this proceedings we describe several strategies for addressing the requirements for high throughput without labeling via: dimensional scaling, rare species targeting and sorting from a stable state. These three approaches are demonstrated through a combination of optical and ultrasonic forces. By combining mostly conservative and non-conservative forces from two different modalities it is possible to reduce the influence of flow velocity on sorting efficiency, hence increasing robustness and scalability. One such approach can be termed "optically enhanced acoustophoresis" which combines the ability of acoustics to handle large volumes of analyte with the high specificity of optical sorting.

Citation Download Citation

Craig McDougall, Paul O'Mahoney, Alan McGuinn, Nicholas A. Willoughby, Yongqiang Qiu, Christine E. M. Demore, and Michael P. MacDonald "Optically enhanced acoustophoresis", Proc. SPIE 10347, Optical Trapping and Optical Micromanipulation XIV, 103471B (25 August 2017); https://doi.org/10.1117/12.2276323

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