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12 July 2017 Ultrafast video imaging of cell division from zebrafish egg using multimodal microscopic system
Sung-Ho Lee, Bumjoon Jang, Dong Hee Kim, Chang Hyun Park, Gyuri Bae, Seung Woo Park, Seung-Han Park
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Proceedings Volume 10324, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017; 103240B (2017) https://doi.org/10.1117/12.2270692
Event: International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017, 2017, Jeju, Korea, Republic of
Abstract
Unlike those of other ordinary laser scanning microscopies in the past, nonlinear optical laser scanning microscopy (SHG, THG microscopy) applied ultrafast laser technology which has high peak powers with relatively inexpensive, low-average-power. It short pulse nature reduces the ionization damage in organic molecules. And it enables us to take bright label-free images. In this study, we measured cell division of zebrafish egg with ultrafast video images using multimodal nonlinear optical microscope. The result shows in-vivo cell division label-free imaging with sub-cellular resolution.
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Sung-Ho Lee, Bumjoon Jang, Dong Hee Kim, Chang Hyun Park, Gyuri Bae, Seung Woo Park, and Seung-Han Park "Ultrafast video imaging of cell division from zebrafish egg using multimodal microscopic system", Proc. SPIE 10324, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017, 103240B (12 July 2017); https://doi.org/10.1117/12.2270692
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KEYWORDS
Ultrafast imaging
Imaging systems
Microscopy
Ionization
Laser applications
Optical microscopy
Second-harmonic generation
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