New tools for confocal macroscopy at OCI

Paul Constantinou

doi:10.1117/12.2283902

29 August 2017 New tools for confocal macroscopy at OCI

Paul Constantinou

Proceedings Volume 10313, Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging; 103132Y (2017) https://doi.org/10.1117/12.2283902
Event: Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging, 2002, Ottawa, Ontario, Canada

Abstract

Light microscopy is a widely used tool in biomedical research. Fluorescence microscopy concentrates on quantitative and qualitative measurements of the fluorescent light emitted from the specimen under study. This is generally done using fluorescent molecules that can be tagged to antibodies, giving specific information about the micro-environment of the sample, i.e. oxygen concentration (tissue hypoxia), and/or to visualize specific structures, such as, tissue morphology (H & E), and blood vessel location (CD31). Biological applications of fluorescence microscopy such as imaging cut and stained tissue/tumour sections use specimens that 'overfill' the field of view of standard microscope objectives. An average tumour in these studies is 5-10 mm wide, while microscope objectives range in their field of view from -1 mm down to a few hundred microns, with smaller fields as magnification power increases. This can pose some difficulties for studies that look at the expression of a parameter across the entire specimen.

Citation Download Citation

Paul Constantinou "New tools for confocal macroscopy at OCI", Proc. SPIE 10313, Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging, 103132Y (29 August 2017); https://doi.org/10.1117/12.2283902

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