Bioluminescent pathogens as a tool to monitor infection in live animals

Lubov Y. Brovko

doi:10.1117/12.2283900

29 August 2017 Bioluminescent pathogens as a tool to monitor infection in live animals

Lubov Y. Brovko

Proceedings Volume 10313, Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging; 103132W (2017) https://doi.org/10.1117/12.2283900
Event: Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging, 2002, Ottawa, Ontario, Canada

Abstract

The study of pathogenic processes is mostly limited to in vitro assays, cell-culture techniques and post mortem examination of infected animals. A better understanding of the infectious process, efficiency of antimicrobial and antibiotic treatment as well as immunomodulatory effects of different food supplements could be achieved by in vivo real-time monitoring of bacterial colonization in live animals. It was proposed recently to use bacterial pathogens with luminescent or fluorescent phenotypes for photonic detection of bacterial cells in living hosts. 14 It was shown that both bacteria transformed with full cassette of luminescent genes from Xenorhabdus luminescens and with Green Fluorescent Protein (GFP) could be visualized in animal using whole-body luminescent or fluorescent imaging techniques with high sensitivity and in real time. We used this approach to investigate the effect of diet on the time-course of infection in mice orally infected with bioluminescent strain of Salmonella enteritidis.

Citation Download Citation

Lubov Y. Brovko "Bioluminescent pathogens as a tool to monitor infection in live animals", Proc. SPIE 10313, Opto-Canada: SPIE Regional Meeting on Optoelectronics, Photonics, and Imaging, 103132W (29 August 2017); https://doi.org/10.1117/12.2283900

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