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The presence of antioxidant issues such as redox potential imbalance in human body is a very important question for modern clinical diagnostics. Implementation of fluorescence analysis into optical diagnostics of such wide distributed in a human body antioxidant as ubiquinone is one of the steps for development of the device with a view to clinical diagnostics of redox potential. Recording of fluorescence was carried out with spectrometer using UV irradiation source with thin band (max at 287 and 330 nm) as a background radiation. Concentrations of ubiquinone from 0.25 to 2.5 mmol/l in explored samples were used for investigation. Recording data was processed using correlation analysis and differential analytical technique. The fourth derivative spectrum of fluorescence spectrum provided the basis for a multicomponent analysis of the solutions. As a technique in clinical diagnostics fluorescence analysis with processing method including differential spectrophotometry, it is step forward towards redox potential calculation and quality control in pharmacy for better health care.
Elvira O. Timofeeva,Elena V. Gorbunova, andAleksandr N. Chertov
"Fluorescence analysis of ubiquinone and its application in quality control of medical supplies", Proc. SPIE 10072, Optical Diagnostics and Sensing XVII: Toward Point-of-Care Diagnostics, 100720N (17 February 2017); https://doi.org/10.1117/12.2252879
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Elvira O. Timofeeva, Elena V. Gorbunova, Aleksandr N. Chertov, "Fluorescence analysis of ubiquinone and its application in quality control of medical supplies," Proc. SPIE 10072, Optical Diagnostics and Sensing XVII: Toward Point-of-Care Diagnostics, 100720N (17 February 2017); https://doi.org/10.1117/12.2252879