Fast quantitative retardance imaging of biological samples using quandri-wave interferometry (Conference Presentation)

Sherazade Aknoun; Julien Savatier; Pierre Bon; Benoit Wattellier; Serge Monneret

doi:10.1117/12.2251975

24 April 2017 Fast quantitative retardance imaging of biological samples using quandri-wave interferometry (Conference Presentation)

Sherazade Aknoun, Julien Savatier, Pierre Bon, Benoit Wattellier, Serge Monneret

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Proceedings Volume 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV; 1006802 (2017) https://doi.org/10.1117/12.2251975
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We describe a technique based on the use of a high-resolution quadri-wave lateral shearing interferometer to perform quantitative linear birefringence measurements on biological samples [1] such as living cells and tissues. The system combines QPI with different excitation polarizations to create retardance images. This creates a new kind of image contrast based on the local retardance, reveals the structure of sample anisotropic components and adds specificity to label-free phase images. We implemented this technique allowing us to take retardance images in less than 1 second which allows us to make high speed acquisitions to reconstruct tissues virtual slides with different modalities (i.e intensity, phase and retardance). Comparisons between healthy and tumoral 10 µm thick skin tissues and collagen orientation studies in the latter will be presented. [1] S. Aknoun, P. Bon, J. Savatier, B. Wattellier, and S. Monneret, "Quantitative retardance imaging of biological samples using quadriwave lateral shearing interferometry," Opt. Express 23, 16383-16406 (2015).

Conference Presentation

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Sherazade Aknoun, Julien Savatier, Pierre Bon, Benoit Wattellier, and Serge Monneret "Fast quantitative retardance imaging of biological samples using quandri-wave interferometry (Conference Presentation)", Proc. SPIE 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV, 1006802 (24 April 2017); https://doi.org/10.1117/12.2251975

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KEYWORDS

Tissues

Interferometry

Birefringence

Collagen

Polarization

Shearing interferometers

Skin

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