Observation of the immune response of cells and tissue through multimodal label-free microscopy

Nicolas Pavillon; Nicholas I. Smith

doi:10.1117/12.2251960

20 February 2017 Observation of the immune response of cells and tissue through multimodal label-free microscopy

Nicolas Pavillon, Nicholas I. Smith

Proceedings Volume 10065, Biophotonics and Immune Responses XII; 100650F (2017) https://doi.org/10.1117/12.2251960
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We present applications of a label-free approach to assess the immune response based on the combination of interferometric microscopy and Raman spectroscopy, which makes it possible to simultaneously acquire morphological and molecular information of live cells. We employ this approach to derive statistical models for predicting the activation state of macrophage cells based both on morphological parameters extracted from the high-throughput full-field quantitative phase imaging, and on the molecular content information acquired through Raman spectroscopy. We also employ a system for 3D imaging based on coherence gating, enabling specific targeting of the Raman channel to structures of interest within tissue.

Citation Download Citation

Nicolas Pavillon and Nicholas I. Smith "Observation of the immune response of cells and tissue through multimodal label-free microscopy", Proc. SPIE 10065, Biophotonics and Immune Responses XII, 100650F (20 February 2017); https://doi.org/10.1117/12.2251960

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