Microsphere microscopic imaging with the coherent light

Sha Guo; Yunxin Wang; Dayong Wang; Qiaowen Lin; Lu Rong

doi:10.1117/12.2243290

31 October 2016 Microsphere microscopic imaging with the coherent light

Sha Guo, Yunxin Wang, Dayong Wang, Qiaowen Lin, Lu Rong

Proceedings Volume 10022, Holography, Diffractive Optics, and Applications VII; 100221N (2016) https://doi.org/10.1117/12.2243290
Event: SPIE/COS Photonics Asia, 2016, Beijing, China

Abstract

The microsphere has great potential to improve the resolution of the system. The high frequency information of the object can be collected by the microsphere to enhance the imaging resolution. However, the unavoidable chromatic dispersion exists in the microsphere incoherence imaging, which reduces the image quality. In this paper, the microsphere microscopy system is designed with the coherent light. The polystyrene (PS) microspheres with the diameter of 50μm and 90μm are applied, and their refractive index is 1.59. The object is a transmission grating with a cycle of 1.2μm and line spacing of 600 nm. The result indicates that the grating can be clearly detected, and the magnification of the microsphere with the diameter of 50μm and 90μm is 2.33 and 1.96 respectively. Comparing with the traditional white light microscopy, the imaging contrast has been improved though the speckle noise is introduced for coherent light. Therefore the microsphere has the potential to improve the resolution of the phase-contrast imaging.

Citation Download Citation

Sha Guo, Yunxin Wang, Dayong Wang, Qiaowen Lin, and Lu Rong "Microsphere microscopic imaging with the coherent light", Proc. SPIE 10022, Holography, Diffractive Optics, and Applications VII, 100221N (31 October 2016); https://doi.org/10.1117/12.2243290

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