Biochemical Applications Of 3-Dimensional Fluorescence Spectrometry

Marc J.P. Leiner; Otto S Wolfbeis

doi:10.1117/12.945377

24 June 1988 Biochemical Applications Of 3-Dimensional Fluorescence Spectrometry

Marc J.P. Leiner, Otto S Wolfbeis

Proceedings Volume 0909, Time-Resolved Laser Spectroscopy in Biochemistry; (1988) https://doi.org/10.1117/12.945377
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

We investigated the 3-dimensional fluorescence of complex mixtures of bioloquids such as human serum, serum ultrafiltrate, human urine, and human plasma low density lipoproteins. The total fluorescence of human serum can be divided into a few peaks. When comparing fluorescence topograms of sera, from normal and cancerous subjects, we found significant differences in tryptophan fluorescence. Although the total fluorescence of human urine can be resolved into 3-5 distinct peaks, some of them. do not result from single fluorescent urinary metabolites, but rather from. several species having similar spectral properties. Human plasma, low density lipoproteins possess a native fluorescence that changes when submitted to in-vitro autoxidation. The 3-dimensional fluorescence demonstrated the presence of 7 fluorophores in the lipid domain, and 6 fluorophores in the protein. dovain- The above results demonstrated that 3-dimensional fluorescence can resolve the spectral properties of complex ,lxtures much better than other methods. Moreover, other parameters than excitation and emission wavelength and intensity (for instance fluorescence lifetime, polarization, or quenchability) may be exploited to give a multidl,ensio,a1 matrix, that is unique for each sample. Consequently, 3-dimensio:Hhal fluorescence as such, or in combination with separation techniques is therefore considered to have the potential of becoming a useful new H.ethod in clinical chemistry and analytical biochemistry.

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Marc J.P. Leiner and Otto S Wolfbeis "Biochemical Applications Of 3-Dimensional Fluorescence Spectrometry", Proc. SPIE 0909, Time-Resolved Laser Spectroscopy in Biochemistry, (24 June 1988); https://doi.org/10.1117/12.945377

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