Laser Light Induced Photosensitization Of Lymphomas Cells And Normal Bone Marrow Cells

Kirpal S Gulliya; Shazib Pervaiz; Don G Nealon; David L VanderMeulen

doi:10.1117/12.945310

15 June 1988 Laser Light Induced Photosensitization Of Lymphomas Cells And Normal Bone Marrow Cells

Kirpal S Gulliya, Shazib Pervaiz, Don G Nealon, David L VanderMeulen

Proceedings Volume 0907, Laser Surgery: Characterization and Therapeutics; (1988) https://doi.org/10.1117/12.945310
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

Dye mediated, laser light induced photosensitization was tested in an in vitro model for its efficacy in eliminating the contaminating tumor cells for ex vivo autologous bone marrow purging. Daudi and U-937 cells (3 x 106/ml) in RPMI-1640 supplemented with 0.25% human albumin were mixed with 20 µg/ml and 25 µg/ml of MC-540, respectively. These cell-dye mixtures were then exposed to 514 nm argon laser light. Identical treatment was given to the normal bone marrow cells. Viability was determined by the trypan blue exclusion method. Results show that at 31.2 J/cm² irradiation, 99.9999% Daudi cells were killed while 87% of the normal bone marrow cells survived. No regrowth of Daudi cells was observed for 30 days in culture. However, a light dose of 93.6 J/cm² was required to obtain 99.999% U-937 cell kill with 80% normal bone marrow cell survival. Mixing of irradiated bone marrow cells with an equal number of lymphoma cells did not interfere with the photodynamic killing of lymphoma cells. Exposure of cells to low doses of recombinant interferon-alpha prior to photodynamic therapy increased the viability of lymphoma cells.

Citation Download Citation

Kirpal S Gulliya, Shazib Pervaiz, Don G Nealon, and David L VanderMeulen "Laser Light Induced Photosensitization Of Lymphomas Cells And Normal Bone Marrow Cells", Proc. SPIE 0907, Laser Surgery: Characterization and Therapeutics, (15 June 1988); https://doi.org/10.1117/12.945310

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