Applications Of Fluorescence Microscopy In Three Dimensions/Microtomoscopy

Ernst H. K. Stelzer; Roelof W . Wijnaendts Van Resandt

doi:10.1117/12.956293

7 July 1986 Applications Of Fluorescence Microscopy In Three Dimensions/Microtomoscopy

Ernst H. K. Stelzer, Roelof W . Wijnaendts Van Resandt

Proceedings Volume 0602, Biostereometrics '85; (1986) https://doi.org/10.1117/12.956293
Event: 1985 International Technical Symposium/Europe, 1985, Cannes, France

Abstract

A confocal scanning laser microscope (CSLM) has been constructed at the EMBL1 and is applied in the investigation of fixed and of living specimens. Contrary to the conventional microscope the CSLM focuses the light emitted by a point source into an object and detects only the light emitted from a small volume in this object. When operated in either fluorescence or reflection mode the CSLM has a true resolution along the optical axis. This feature is used to record independent images at different levels of height in a fluorescently labelled object or to scan the object in a plane parallel to the optical axis.

Citation Download Citation

Ernst H. K. Stelzer and Roelof W . Wijnaendts Van Resandt "Applications Of Fluorescence Microscopy In Three Dimensions/Microtomoscopy", Proc. SPIE 0602, Biostereometrics '85, (7 July 1986); https://doi.org/10.1117/12.956293

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