On The Water Subtraction In The Fourier Transform Infrared (FTIR) Spectra Of Proteins And Lipids

Marie Therrien; Michel Lafleur; Michel Pezolet

doi:10.1117/12.970757

20 December 1985 On The Water Subtraction In The Fourier Transform Infrared (FTIR) Spectra Of Proteins And Lipids

Marie Therrien, Michel Lafleur, Michel Pezolet

Proceedings Volume 0553, Fourier and Computerized Infrared Spectroscopy; (1985) https://doi.org/10.1117/12.970757
Event: 1985 International Conference on Fourier and Computerized Infrared Spectroscopy, 1985, Ottawa, Canada

Abstract

A procedure is described to subtract the buffer contribution from the infrared spectra of aqueous solutions of proteins using the 2130 cm-1 association band of water as an internal intensity standard. After correction, the spectra of several globular proteins were used to develop a method to evaluate their conformation in solution.

Citation Download Citation

Marie Therrien, Michel Lafleur, and Michel Pezolet "On The Water Subtraction In The Fourier Transform Infrared (FTIR) Spectra Of Proteins And Lipids", Proc. SPIE 0553, Fourier and Computerized Infrared Spectroscopy, (20 December 1985); https://doi.org/10.1117/12.970757

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