Holographic motion picture microscopy

C. Knox; R. E. Brooks

doi:10.1117/12.953475

1 June 1971 Holographic motion picture microscopy

C. Knox, R. E. Brooks

Proceedings Volume 0024, Underwater Photo-Optical Instrumentation Applications III; (1971) https://doi.org/10.1117/12.953475
Event: Underwater Photo-Optical Instrumentation Applications, 1971, Honolulu, United States

Abstract

Holographic motion pictures provide a means for recording and observing transient, dynamic, small-scale events occurring at unpredictable times and locations in a given volume of space. Using an argon ion laser to generate repetitive pulses of 50 μs duration at a wavelength of 514.5 nm (5145 Å), holographic movies of living marine plankton organisms were produced by recording a series of in-line holograms at 70 frames/s on 35 mm film. The reconstructed holographic images may be viewed with a standard microscope, photographed with a still camera, or copied with a conventional movie camera for subsequent projection. Image resolution of 10 μm was obtained.

Citation Download Citation

C. Knox and R. E. Brooks "Holographic motion picture microscopy", Proc. SPIE 0024, Underwater Photo-Optical Instrumentation Applications III, (1 June 1971); https://doi.org/10.1117/12.953475

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