Computational phase imaging for label-free 3D microscopy: noninterferometic phase retrieval and intensity diffraction tomography

Chao Zuo

doi:10.1117/12.3016413

18 June 2024 Computational phase imaging for label-free 3D microscopy: noninterferometic phase retrieval and intensity diffraction tomography

Chao Zuo

Author Affiliations +

Proceedings Volume PC12996, Unconventional Optical Imaging IV; PC1299608 (2024) https://doi.org/10.1117/12.3016413
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Nowadays, fluorescence microscopy has made the leap from 2D to 3D or even 4D (xyz+t) imaging. On the other hand, Zernike phase contrast microscopy, which was awarded the Nobel Prize in Physics in 1953, has become the standard feature for modern biological microscopy, but is still limited to 2D imaging. Currently, life science research urgently needs a new "label-free 3D microscopy" mode that complements confocal/two-photon/super-resolution 3D fluorescence microscopy technology to meet the needs of rapid, high-resolution, long-term imaging of live cells in 3D. In this talk, we will present some of our research progress in "noninterferometic" intensity diffraction tomography, including: quantitative phase imaging and diffraction tomography based on transport of intensity and Fourier ptychography. Our results highlight a new era in which strict coherence and interferometry are no longer prerequisites for quantitative phase imaging and diffraction tomography, paving the way toward new generation label-free three-dimensional microscopy, with applications in all branches of biomedicine.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Chao Zuo "Computational phase imaging for label-free 3D microscopy: noninterferometic phase retrieval and intensity diffraction tomography", Proc. SPIE PC12996, Unconventional Optical Imaging IV, PC1299608 (18 June 2024); https://doi.org/10.1117/12.3016413

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