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13 March 2024 High-resolution, high-throughput lensless coded ptychography for biomedical imaging
Guoan Zheng
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Proceedings Volume PC12852, Quantitative Phase Imaging X; PC1285204 (2024) https://doi.org/10.1117/12.3000541
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
We discuss a coded ptychography technique that significantly enhances imaging resolution and throughput, achieving an order of magnitude improvement over previous methods. Our platform involves translating samples across disorder-engineered surfaces for lensless diffraction data acquisition. These engineered surfaces can be created by smearing a monolayer of blood on the image sensor. By monitoring the phase wraps of the recovered images, we have successfully observed bacterial growth at 15-second intervals over a 120 mm^2 area, achieving phase sensitivity comparable to interferometric measurements. For drug-screening applications, we demonstrate proof-of-concept rapid antibiotic susceptibility testing. By adopting a depth-multiplexed configuration, our approach can be extended to simultaneously image a stack of biospecimens. The fusion of high phase sensitivity, superior spatiotemporal resolution, an ultra-large field of view, and depth-multiplexing capability sets the coded ptychographic microscopy apart from existing imaging techniques, marking an important advancement for high-throughput label-free lensless imaging.
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Guoan Zheng "High-resolution, high-throughput lensless coded ptychography for biomedical imaging", Proc. SPIE PC12852, Quantitative Phase Imaging X, PC1285204 (13 March 2024); https://doi.org/10.1117/12.3000541
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KEYWORDS
Biomedical optics
Biological samples
Image resolution
Laser optics
Optical microscopy
Light sources
Light sources and illumination
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