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16 March 2023 Optical gearbox enabled high-speed laser scanning multiphoton microscopy (Conference Presentation)
Jianian Lin, Zongyue Cheng, Meng Cui
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Proceedings Volume PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII; PC1239008 (2023) https://doi.org/10.1117/12.2650074
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Laser scanning multiphoton microscopy is widely used for in vivo imaging applications. Commonly employed systems can offer an imaging rate of tens of Hz. With the emergence of high-speed function indicators, an imaging rate at hundreds of Hz is required. Recently, we developed the optical gearbox, which can convert commonly employed imaging systems and sources for high-speed imaging with a frame rate ranging from tens of Hz to 1,000 Hz. Here, we present our latest work which showcases the performance of optical gearbox based two-photon laser scanning microscopy for in vivo imaging of neuronal and vascular dynamics in mouse brain.
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© (2023) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
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Jianian Lin, Zongyue Cheng, and Meng Cui "Optical gearbox enabled high-speed laser scanning multiphoton microscopy (Conference Presentation)", Proc. SPIE PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII, PC1239008 (16 March 2023); https://doi.org/10.1117/12.2650074
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KEYWORDS
Laser scanners
Multiphoton microscopy
Imaging systems
In vivo imaging
Brain
High speed imaging
Microscopy
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