3D pathology of cleared clinical specimens using an optimized nonorthogonal dual-objective (NODO) open-top light-sheet (OTLS) microscope (Conference Presentation)

Kevin W. Bishop; Adam K. Glaser; Lindsey A. Barner; Robert B. Serafin; Jonathan T. C. Liu

doi:10.1117/12.2648611

15 March 2023 3D pathology of cleared clinical specimens using an optimized nonorthogonal dual-objective (NODO) open-top light-sheet (OTLS) microscope (Conference Presentation)

Kevin W. Bishop, Adam K. Glaser, Lindsey A. Barner, Robert B. Serafin, Jonathan T. C. Liu

Author Affiliations +

Proceedings Volume PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX; PC1238504 (2023) https://doi.org/10.1117/12.2648611
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Open-Top Light-Sheet (OTLS) microscopy is an emerging technique for cleared-tissue imaging that alleviates many of the physical constraints on sample size and geometry associated with conventional light-sheet microscopes. Existing OTLS designs generally use either two orthogonal objectives or a single shared objective for illumination and collection, however these architectures have various limitations for moderate NA cleared-tissue imaging. We previously developed an alternative Nonorthogonal Dual-Objective (NODO) OTLS configuration with a number of advantages for moderate-NA imaging of cleared tissues. Here we describe our latest NODO system, which is optimized specifically for high-throughput imaging of clinical samples for 3D pathology.

Conference Presentation

Citation Download Citation

Kevin W. Bishop, Adam K. Glaser, Lindsey A. Barner, Robert B. Serafin, and Jonathan T. C. Liu "3D pathology of cleared clinical specimens using an optimized nonorthogonal dual-objective (NODO) open-top light-sheet (OTLS) microscope (Conference Presentation)", Proc. SPIE PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, PC1238504 (15 March 2023); https://doi.org/10.1117/12.2648611

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