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3 October 2022 Enhanced total Internal reflection fluorescence imaging of viruses
Anita Mouttou, Fabien Le Marchand, Manon Gourdelier, Antonin Moreau, Delphine Muriaux, Julien Lumeau, Cyril Favard, Aude L. Lereu
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Proceedings Volume PC12196, Active Photonic Platforms 2022; PC121961H (2022) https://doi.org/10.1117/12.2632933
Event: SPIE Nanoscience + Engineering, 2022, San Diego, California, United States
Abstract
TIRF-Microscopy is widely used in cell biology to monitor dynamic biomolecular events occurring at the cell membranes. However, this technic is limited by weak fluorescence signal and background noise degrading the spatial resolution. We present high sensitive fluorescent TIRF-M using glass coverslips coated with one-dimensional photonic crystal (1DPC). The latter is made of dielectric multilayers optimized to generate a large field enhancement under TIR illumination at its free interface. Using a TIRF-microscope, we successfully demonstrate the capability of these substrates to enhance the fluorescence signal as compared to classical glass coverslips over fluorescently labeled HIV-1 and SARS-CoV-2 virus-like-particles imaging.
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Anita Mouttou, Fabien Le Marchand, Manon Gourdelier, Antonin Moreau, Delphine Muriaux, Julien Lumeau, Cyril Favard, and Aude L. Lereu "Enhanced total Internal reflection fluorescence imaging of viruses", Proc. SPIE PC12196, Active Photonic Platforms 2022, PC121961H (3 October 2022); https://doi.org/10.1117/12.2632933
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KEYWORDS
Luminescence
Viruses
Adaptive optics
Glasses
Microscopes
Microscopy
Particles
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