Raman flow cytometry on a chip

Kotaro Hiramatsu

doi:10.1117/12.2615334

2 March 2022 Raman flow cytometry on a chip

Kotaro Hiramatsu

Proceedings Volume PC11971, High-Speed Biomedical Imaging and Spectroscopy VII; PC119710L (2022) https://doi.org/10.1117/12.2615334
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Raman flow cytometry is a promising approach for large-scale single-cell analysis in a label-free manner. However, due to the limited sensitivity of Raman spectral measurements, its application range is still limited compared to fluorescence-based flow cytometry. Here we present the developments of a high-throughput Raman-based spectroscopic flow cytometer and a Raman-activated cell sorter realized by integrating state-of-the-art coherent Raman techniques and acoustofluidic devices for cell manipulation in a flow stream. We demonstrate analysis and sorting of microalgal cells based on their bioproducts’ contents such as palamyron, starch, and carotenoids.

Conference Presentation

Citation Download Citation

Kotaro Hiramatsu "Raman flow cytometry on a chip", Proc. SPIE PC11971, High-Speed Biomedical Imaging and Spectroscopy VII, PC119710L (2 March 2022); https://doi.org/10.1117/12.2615334

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