Autofluorescence lifetime imaging of single cell metabolism

Melissa C. Skala

doi:10.1117/12.2685585

13 March 2024 Autofluorescence lifetime imaging of single cell metabolism

Melissa C. Skala

Proceedings Volume PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; PC1285405 (2024) https://doi.org/10.1117/12.2685585
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We demonstrate optical redox ratio and fluorescence lifetime imaging microscopy (FLIM) of intrinsic metabolic co-factors NAD(P)H and FAD to quantify single cell metabolism and function. This approach is attractive because it does not require cell surface labels or transfection, enabling rapid assessment of dynamic events. Multiphoton microscopy provides near infrared excitation of these autofluorescent molecules, thereby maximizing cell viability. Newly trained neural networks automatically segment single cells for analysis of heterogeneity within and between patients. Overall, this approach is attractive for both basic research and patient management in cancer and immunology.

Conference Presentation

Citation Download Citation

Melissa C. Skala "Autofluorescence lifetime imaging of single cell metabolism", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC1285405 (13 March 2024); https://doi.org/10.1117/12.2685585

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