Dual-modal optical projection tomography microscopy for cancer diagnosis

Qin Miao; Julia Yu; Michael G. Meyer; J. Richard Rahn; Thomas Neumann; Alan C. Nelson; Eric J. Seibel

doi:10.1117/12.839824

24 February 2010 Dual-modal optical projection tomography microscopy for cancer diagnosis

Qin Miao, Julia Yu, Michael G. Meyer, J. Richard Rahn, Thomas Neumann, Alan C. Nelson, Eric J. Seibel

Proceedings Volume 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII; 75700H (2010) https://doi.org/10.1117/12.839824
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

A dual-modal optical projection tomography microscope (OPTM) is presented, which produces three-dimensional images of single cells with isometric high resolution both in fluorescence and absorption mode. Depth of field of a high numerical aperture objective is extended by scanning the focal plane through the sample in order to enable reconstruction by back-projection method. Cells are fixed, stained, and mixed with optical gel and injected into the capillary for imaging. Combining absorption and fluorescence mode allows us to image different aspects of the disease process. Images of cells stained with both hematoxylin and fluorescence probes are shown. Registrations between two modes are discussed.

Citation Download Citation

Qin Miao, Julia Yu, Michael G. Meyer, J. Richard Rahn, Thomas Neumann, Alan C. Nelson, and Eric J. Seibel "Dual-modal optical projection tomography microscopy for cancer diagnosis", Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 75700H (24 February 2010); https://doi.org/10.1117/12.839824

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