Simulation Tests Of Restoring Truncated Fluorescence Micrographs

Timothy J. Holmes; Yi-Hwa Liu

doi:10.1117/12.962699

22 December 1989 Simulation Tests Of Restoring Truncated Fluorescence Micrographs

Timothy J. Holmes, Yi-Hwa Liu

Proceedings Volume 1161, New Methods in Microscopy and Low Light Imaging; (1989) https://doi.org/10.1117/12.962699
Event: 33rd Annual Technical Symposium, 1989, San Diego, United States

Abstract

The results of a computer simulation of restoring truncated fluorescence micrographs is presented. The particular application that is considered is that of three-dimensional (3-D) optical sectioning with a conventional microscope and a photon-counting camera.1,2 Practicalities that are considered in the simulation include quantum-photon noise, the degrading effects of the missing cone in the 3-D optical transfer function (OTF), and the effects of a truncated image. The restoration algorithT used, based on maximum-likelihood estimation, has been presented previously in a nonregularized4form. Herein we present an adaptation of a regularization method developed by others for positron-emission tomography. The restored images indicate that it may be feasible to use this method for optical sectioning with a conventional microscope. We suggest that this might provide an economical alternative to optical sectioning with a confocal microscope.

Citation Download Citation

Timothy J. Holmes and Yi-Hwa Liu "Simulation Tests Of Restoring Truncated Fluorescence Micrographs", Proc. SPIE 1161, New Methods in Microscopy and Low Light Imaging, (22 December 1989); https://doi.org/10.1117/12.962699

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