Correlative Brillouin microscopy and optical diffraction tomography for quantitative characterization of mechanical and biophysical properties of biological samples (Conference Presentation)

Kyoohyun Kim; Raimund Schlüßler; Timon Beck; Salvatore Girardo; Jochen R. Guck

doi:10.1117/12.2546448

11 March 2020 Correlative Brillouin microscopy and optical diffraction tomography for quantitative characterization of mechanical and biophysical properties of biological samples (Conference Presentation)

Kyoohyun Kim, Raimund Schlüßler, Timon Beck, Salvatore Girardo, Jochen R. Guck

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Proceedings Volume 11249, Quantitative Phase Imaging VI; 112490O (2020) https://doi.org/10.1117/12.2546448
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We present a combined optical system for Brillouin microscopy with optical diffraction tomography (ODT), which can reconstruct the three-dimensional refractive index (RI) distribution of biological samples. By correlating Brillouin frequency shift with the reconstructed RI distribution in the same field-of-view, we can calculate the precise longitudinal modulus of the samples without a priori information. We demonstrate the capabilities of the method using a cell phantom consisting of different hydrogel beads with known mechanical properties, and apply it to the quantitative characterization of the mechanical and biophysical properties of nuclear compartments inside individual cells under various physiological conditions including cell cycle progression and drug treatments.

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Kyoohyun Kim, Raimund Schlüßler, Timon Beck, Salvatore Girardo, and Jochen R. Guck "Correlative Brillouin microscopy and optical diffraction tomography for quantitative characterization of mechanical and biophysical properties of biological samples (Conference Presentation)", Proc. SPIE 11249, Quantitative Phase Imaging VI, 112490O (11 March 2020); https://doi.org/10.1117/12.2546448

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KEYWORDS

Microscopy

Biomedical optics

Diffraction

Optical microscopy

Optical tomography

Tomography

Phase measurement

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