Biomedical Applications in Molecular, Structural, and Functional Imaging

Multipurpose nonlinear optical imaging system for in vivo and ex vivo multimodal histology

[+] Author Affiliations
Martin Weinigel

JenLab GmbH, Schillerstrasse 1, Jena 07745, Germany

Hans Georg Breunig, Aisada Uchugonova, Karsten König

JenLab GmbH, Schillerstrasse 1, Jena 07745, Germany

Saarland University, Department of Biophotonics and Laser Technology, Campus A5.1, Saarbrücken 66123, Germany

J. Med. Imag. 2(1), 016003 (Mar 04, 2015). doi:10.1117/1.JMI.2.1.016003
History: Received November 29, 2014; Accepted February 6, 2015
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Abstract.  We report on a flexible multipurpose nonlinear microscopic imaging system based on a femtosecond excitation source and a photonic crystal fiber with multiple miniaturized time-correlated single-photon counting detectors. The system provides the simultaneous acquisition of e.g., two-photon autofluorescence, second-harmonic generation, and coherent anti-Stokes Raman scattering images. Its flexible scan head permits ex vivo biological imaging with subcellular resolution such as rapid biopsy examination during surgery as well as imaging on small as well as large animals. Above all, such an arrangement perfectly matches the needs for the clinical investigation of human skin in vivo where knowledge about the distribution of endogenous fluorophores, second-harmonic generation–active collagen as well as nonfluorescent lipids is of high interest.

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© 2015 Society of Photo-Optical Instrumentation Engineers

Citation

Martin Weinigel ; Hans Georg Breunig ; Aisada Uchugonova and Karsten König
"Multipurpose nonlinear optical imaging system for in vivo and ex vivo multimodal histology", J. Med. Imag. 2(1), 016003 (Mar 04, 2015). ; http://dx.doi.org/10.1117/1.JMI.2.1.016003


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